Two years after a clash of egos forced Mystery, Inc. to close its doors, Scooby-Doo and his crime-solving cohorts are individually summoned to Spooky Island to investigate a series of paranormal incidents at the ultra-hip spring break hot spot. Concerned his park might truly be haunted, the owner tries to reunite those notoriously meddling detectives to sovle the mystery before his supernatural secret scares away the college crowd.

The gang abandons their plans for a relaxing vacation when a scaly sea serpent surfaces.